RNA-Seq Study of Microbially Induced Hemocyte Transcripts from Larval Heliothis virescens (Lepidoptera: Noctuidae)

Shelby: Induction of Tobacco Budworm Antimicrobial Peptides 921 INDUCTION OF ANTIMICROBIAL PEPTIDES IN INFECTED TISSUES OF LARVAL HELIOTHIS VIRESCENS (LEPIDOPTERA: NOCTUIDAE)

Effective biological control of destructive heliothine larvae via application of microbial entomopathogens or parasitoids is limited by a robust immune response against infection and parasitization. To better understand the immune response of budworms against microbial entomopathogens, several orthologs of antibacterial response proteins were identified and extracted from a tobacco budworm, Hel...

Spermiogenesis of Eupyrene Sperm in Prepupae"} Pupae"} and Adults of Heliothis virescens (Lepidoptera: Noctuidae): An Ultrastructural Study

Immunolocalization of the 17 kDa vacuolar H(+)-ATPase subunit c in Heliothis virescens midgut and malpighian tubules with an anti-peptide antibody.

The transmembrane sector of V-ATPases is involved in proton conduction across the membrane where a 15-17 kDa proteolipid forms a putative proton channel. An affinity-purified rabbit polyclonal antibody was developed to an antigenic and putatively extracellular region of a cloned 17 kDa proteolipid. In larval tissue sections, this antibody labeled the midgut goblet cell apical membrane in Heliot...

Baculovirus Induced Transcripts in Hemocytes from the Larvae of Heliothis virescens

Using RNA-seq digital difference expression profiling methods, we have assessed the gene expression profiles of hemocytes harvested from Heliothis virescens that were challenged with Helicoverpa zea single nucleopolyhedrovirus (HzSNPV). A reference transcriptome of hemocyte-expressed transcripts was assembled from 202 million 42-base tags by combining the sequence data of all samples, and the a...

Response of Heliothis virescens (Lepidoptera: Noctuidae) strains to Bacillus thuringiensis Cry1Ac incorporated into different insect artificial diets.

Susceptibility to the Cry1Ac toxin from Bacillus thuringiensis in tobacco budworm, Heliothis virescens (F.) (Lepidoptera: Noctuidae), is usually measured by performing bioassays under laboratory conditions. Accurate comparison of Cry1Ac susceptibility among H. virescens samples conducted in different places is challenged by several important methodological aspects, especially if different insec...